Synapsin (phospho Ser549) Antibody

• Background:

  Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996) . This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002) . Ser 549 along with Ser 62 and Ser 67 are the sites of synapsin I that are phosphorylated by MAP kinase (Jovanovic et al., 1996) . Phosphorylation and subsequent dephosphorylation of this site is thought to play a key role in synaptic vesicle trafficking.

• NCBI Gene ID:

  281510

• Swiss Prot:

  P17599

• Accession Number:

  P17599

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog, Bovine

• Immunogen:

  Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser549 of synapsin I.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Neuroscience

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Synapsin antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

• Calculated Molecular Weight:

  78

• Applications Notes:

  The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with human, mouse, non-human primate, bovine and canine tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

• Symbol:

  SYN1

• NCBI Official Name:

  Synapsin I

• NCBI Organism:

  Bos taurus

• Background Reference 01:

  Jovanovic JN, Benfenati, F, Siow YL, Sihra TS, Sanghera JS, Pelech SL, Greengard P, Czernik AJ (1996) Neurotrophins stimulate phosphorylation of Synapsin I by MAP kinase and regulate Synapsin I-actin interactions. Neurobiology 93:3679-3683.

• Background Reference 02:

  Czernik AJ, Pang DT, Greengard P (1987) Amino acid sequences surrounding the cAMP-dependent and calcium/calmodulin-dependent phosphorylation sites in rat and bovine synapsin I. Proc Natl Acad Sci (USA) 84:7518-7522.

• Background Reference 03:

  Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. J Neurosci 22:4372-4380.

• Tested Applications:

  WB, IHC

• Protein ID:

  1351166

• Physical Properties:

  Liquid