PLK1 (phospho Thr210) Antibody

• Background:

  Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004) . Deregulated expression of PLK’s is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005) . It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser137 and Thr210 (van de Weerdt et al., 2005) .

• NCBI Gene ID:

  5347

• Swiss Prot:

  P53350

• Accession Number:

  P53350

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog, Zebrafish, Bovine, Frog

• Immunogen:

  Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr210 of PLK1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PLK1 antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

• Calculated Molecular Weight:

  66

• Applications Notes:

  The antibody has been directly tested for reactivity in Western blots in rat and human tissues. It is anticipated that the antibody will react with bovine, canine, mouse, non-human primate, Xenopus and Zebrafish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

• Symbol:

  PLK1

• NCBI Official Name:

  Polo-like kinase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Van Vugt MA, van de Weerdt BC, Vader G, Janssen H, Calafat J, Klompmaker R, Wolthuis RM, Medema RH (2004) Polo-like kinase 1 is required for bipolar spindle formation but is dispesible for anaphase promoting complex cdc20 activation and initiation of cytokinesis. J. Biol. Chem. Aug 27;279 (35) :36841-54.

• Background Reference 02:

  Takai N, Hamanaka R, Yoshimatsu J, Miyakawa I (2005) Polo-like kinases and cancer. Oncogene Jan 10; 24 (2) : 287-91.

• Background Reference 03:

  Van de Weerdt BC, van Vugt MA, Lindon C, Kauw JJ, Rozendaal MJ, Klompmaker R, Wolthuis RM, Medema RH (2005) Uncoupling anaphase-promoting complex/cyclosome activity from spindle assembly checkpoint control by deregulating polo-like kinase 1. Mol. Cell Biol. Mar; 25 (5) :2031-44.

• Tested Applications:

  WB

• Protein ID:

  1709658

• Physical Properties:

  Liquid