HNF4a (phospho Ser304) Antibody

• Background:

  The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

• NCBI Gene ID:

  3172

• Swiss Prot:

  P41235

• Accession Number:

  NP_000448.3

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  HNF4a (Phospho-Ser304) antibody was raised against a peptide sequence around phosphorylation site of serine 304 (L-R-S (p) -Q-V) derived from Human HNF4α .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  52 kDa

• Specificity:

  This antibody detects endogenous level of HNF4α only when phosphorylated at serine 304.

• Applications Notes:

  Western Blot: 1:1000

• Symbol:

  HNF4A

• NCBI Official Name:

  Hepatocyte nuclear factor 4, alpha

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kritis A. A, et al. Gene 173:275-280 (1996) Drewes T, et al. Mol. Cell. Biol. 16:925-931 (1996) Yamagata K, et al. Nature 384:458-460 (1996) Tanaka T, et al. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases

• Other Product Names:

  TCF, HNF4, MODY, MODY1, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, HNF4alpha, HNRPA1, TCF, HNF4A

• Tested Applications:

  WB

• Protein ID:

  31077205

• Physical Properties:

  Liquid