CREB (Ab 129) Antibody

• Background:

  CREB1 is a transcription factor that is a member of the bZIP family of DNA binding proteins. It binds as a homodimer to the CRE (cAMP-Responsive Element), an octameric palindrome containing a conserved core sequence, 5-prime-TGACG-3-prime. It consists of two transcript variants encoding respective isoforms produced by alternate splicing. It is mapped to 2q32.3-q34 . It is phosphorylated by several protein kinases and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB1 is crucial for the consolidation of long-term conditioned fear memories, but not for encoding, storage, or retrieval of these memories. It is required for the stability of reactivated or retrieved conditioned fear memories .

• NCBI Gene ID:

  1385

• Swiss Prot:

  P16220

• Accession Number:

  NP_004370.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  CREB (Ab-129) antibody was raised against a peptide sequence around aa.127~131 (I-L-S-R-R) derived from Human CREB.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis, Cancer, Neuroscience

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  43 kDa

• Specificity:

  This antibody detects endogenous level of total CREB protein.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  CREB1

• NCBI Official Name:

  CAMP responsive element binding protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Chrystelle V. Garat, et al. (2006) Mol. Cell. Biol ; 26: 4934 - 4948. Lilah Rothem, et al. (2004) Mol. Pharmacol ; 66: 1536 - 1543. Darren R. Tyson, et, al. (2002) Endocrinology; 143: 674. Kyung-Woo Park, et al. (2003) Arterioscler. Thromb. Vasc. Biol ; 23: 1364.

• Other Product Names:

  CREB, IKKB, CREB-1, CREB1

• Tested Applications:

  WB, IHC

• Protein ID:

  4758054

• Physical Properties:

  Liquid