CREB (Ab 133) Antibody

• Background:

  This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.

• NCBI Gene ID:

  1385

• Swiss Prot:

  P16220

• Accession Number:

  NP_004370.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  CREB (Ab-133) antibody was raised against a peptide sequence around aa. 131~135 (R-P-S-Y-R) derived from Human CREB.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis, Cancer, Neuroscience

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  43 kDa

• Specificity:

  This antibody detects endogenous level of total CREB protein and it also detects the CREB-related protein ATF-1.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  CREB1

• NCBI Official Name:

  CAMP responsive element binding protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Xing J, et al. (1998) Mol Cell Biol 18 (4) : 1946-55. Tan Y, et al. (1996) EMBO J; 15 (17) : 4629-42. Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385. Mayo LD, et al. (2001) Biol Chem; 276 (27) : 25184-9. Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.

• Other Product Names:

  CREB, NR3C3, CREB-1, CREB1

• Tested Applications:

  WB, IHC

• Protein ID:

  4758054

• Physical Properties:

  Liquid