ADD1 (Ab 726) Antibody

• Background:

  Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma) . Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca (2+) /calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.

• NCBI Gene ID:

  118

• Swiss Prot:

  P35611

• Accession Number:

  NP_001110.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  ADD1 (Ab-726) antibody was raised against a peptide sequence around aa.724~728 (T-P-S-F-L) derived from Human ADD1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  130 kDa

• Specificity:

  This antibody detects endogenous level of total ADD1 protein.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  ADD1

• NCBI Official Name:

  Adducin 1 (alpha)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Pariser H, et al. Proc Natl Acad Sci USA 2005 Aug 30; 102 (35) : 12407-12Tamaru S, et al. Biochem Biophys Res Commun 2005 Jul 01; 332 (2) : 347-51Barkalow KL, et al. J Cell Biol 2003 May 12; 161 (3) : 557-70

• Other Product Names:

  ADDA, ERBB, ERBB1, HER1, ADDA, Erythrocyte adducin alpha subunit

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  29826319

• Physical Properties:

  Liquid