Cdc25C (Ab 216) Antibody

• Background:

  Cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encodedprotein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

• NCBI Gene ID:

  995

• Swiss Prot:

  P30307

• Accession Number:

  NP_001781.2

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Cdc25C (Ab-216) antibody was raised against a peptide sequence around aa.214~218 (S-P-S-M-P) derived from Human cdc25C.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  60 kDa

• Specificity:

  This antibody detects endogenous level of total cdc25C protein.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  CDC25C

• NCBI Official Name:

  Cell division cycle 25C

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Toyoshima-Morimoto F. et al. (2002) EMBO Rep. 3 (4) : 341-348. Ferguson AM. et al. (2005) Mol Cell Biol. 25 (7) : 2853-2860. Donzelli M. et al. (2003) EMBO Rep. 4 (7) : 671-677. Chen F. et al. (2002) Proc Natl Acad Sci U S A. 99 (4) : 1990-1995.

• Other Product Names:

  CDC25, PPP1R60, CREB2, CREBP1, CDC25M1, MPIP3

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  125625350

• Physical Properties:

  Liquid