P53 (Ab 15) Antibody

• Background:

  P53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes

• NCBI Gene ID:

  7157

• Swiss Prot:

  P04637

• Accession Number:

  NP_000537.3

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  P53 (Ab-15) antibody was raised against a peptide sequence around aa.13~17 (P-L-S-Q-E) derived from Human p53.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis, Cancer, Cell Cycle

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  53 kDa

• Specificity:

  This antibody detects endogenous level of total p53 protein.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  TP53

• NCBI Official Name:

  Tumor protein p53

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lin T, et al. (2005) Nat Cell Biol; 7 (2) : 165-71. Vega FM, et al. (2004) Mol Cell Biol; 24 (23) : 10366-80. Li J, et al. (2004) J Biol Chem; 279 (40) : 41275-9. Wang J, et al. (2004) J Biol Chem; 279 (38) : 39584-92.

• Other Product Names:

  P53, BCC7, LFS1, TRP53, GRB1, Tumor suppressor p53, Phosphoprotein p53, Antigen NY-CO-13, TP53

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  120407068

• Physical Properties:

  Liquid