P38 MAPK (phospho Thr180) Antibody

• Background:

  Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.

• NCBI Gene ID:

  1432

• Swiss Prot:

  Q16539

• Accession Number:

  NP_001306.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  P38 MAPK (Phospho-Thr180) antibody was raised against a peptide sequence around phosphorylation site of threonine 180 (E-M-T (p) -G-Y) derived from Human P38MAPK.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  43 kDa

• Specificity:

  This antibody detects endogenous level of P38 MAPK only when phosphorylated at Threonine 180.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  MAPK14

• NCBI Official Name:

  Mitogen-activated protein kinase 14

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kim JE, et al. (2005) J Proteome Res Jul-Aug; 4 (4) : 1339-1346Meng F, et al. (2005) Am J Physiol Cell Physiol May 25Jin ZH, et al. (2005) Oncogene Mar 17; 24 (12) : 1973-1981

• Other Product Names:

  RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, PLC1, MAPK2, MAPKAPK-2, MAPKAPK2

• Tested Applications:

  WB, IHC

• Protein ID:

  4503069

• Physical Properties:

  Liquid