P44 / 42 MAP Kinase (phospho Thr202) Antibody

• Background:

  Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2) . Phosphorylates SPZ1

• NCBI Gene ID:

  5595

• Swiss Prot:

  P27361

• Accession Number:

  NP_001035145.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  P44/42 MAP Kinase (Phospho-Thr202) antibody was raised against a peptide sequence around phosphorylation site of threonine 202 (F-L-T (p) -E-Y) derived from Human p44/42 MAP Kinase.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Apoptosis, Cancer

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  42, 44 kDa

• Specificity:

  This antibody detects endogenous level of p44/42 MAP Kinase only when phosphorylated at threonine 202.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  MAPK3

• NCBI Official Name:

  Mitogen-activated protein kinase 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 162 Jingui Yu, et al. (2005) Anesth Analg 101: 315-321Hironobu Ihn et al. (2000) Immunology 165: 2149-2155

• Other Product Names:

  ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK, PKD, PKD1, PRKCM, ERK, Extracellular signal- regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 1

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  91718897

• Physical Properties:

  Liquid