P44/42 MAP Kinase (Phospho-Thr202) Antibody

• Background:

  Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2) . Phosphorylates SPZ1 .

• Description:

  This is a p44/42 MAP Kinase (Phospho-Thr202) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  ERK; ERT2; Extracellular signal- regulated kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase 1

• Gene ID:

  5595

• Swiss Prot:

  P27361

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Peptide sequence around phosphorylation site of threonine 202 (F-L-T (p) -E-Y) derived from Human p44/42 MAP Kinase.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB, IHC, IF

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:1000, IHC 1:50-1:100, IF 1:100-1:200

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Host or Source:

  Rabbit

• Isotype:

  IgG