IRS 1 (phospho Ser636) Antibody

• Background:

  May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit

• NCBI Gene ID:

  3667

• Swiss Prot:

  P35568

• Accession Number:

  NP_005535.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  IRS-1 (Phospho-Ser636) antibody was raised against a peptide sequence around phosphorylation site of serine 636 (P-M-S (p) -P-K) derived from Human IRS-1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  180 kDa

• Specificity:

  This antibody detects endogenous level of total IRS-1 protein.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  IRS1

• NCBI Official Name:

  Insulin receptor substrate 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98 (8) : 4640-4645Tzatsos A, et al. (2006) Mol Cell Biol; 26 (1) : 63-76Kadowaki T, et al. (2000) J Clin Invest; 106 (4) : 459-465Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98 (8) : 4640-4645Szanto I, et al. (2000) Proc Natl Acad Sci U S A; 97 (5) : 2355-2360

• Other Product Names:

  HIRS-1, CTNNB, OK/SW-cl.35, PRO2286, IRS-1, IRS1

• Tested Applications:

  WB, IHC

• Protein ID:

  5031805

• Physical Properties:

  Liquid