Pyk2 (phospho Tyr402) Antibody

• Background:

  Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.

• NCBI Gene ID:

  2185

• Swiss Prot:

  Q14289

• Accession Number:

  NP_004094.3

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Pyk2 (Phospho-Tyr402) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 402 (D-I-Y (p) -A-E) derived from Human Pyk2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  116 kDa

• Specificity:

  This antibody detects endogenous level of Pyk2 only when phosphorylated at tyrosine 402.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  PTK2B

• NCBI Official Name:

  Protein tyrosine kinase 2 beta

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Gluck SL, et al. (2004) J Clin Invest; 114 (12) : 1696-1699Benzing T, et al. (2001) Proc Natl Acad Sci U S A; 98 (17) : 9784-9789Tian D, et al. (2002) Mol Cell Biol; 22 (8) : 2650-2662Lu Z, et al. (2001) Mol Cell Biol; 21 (12) : 4016-4031Krishnan HH, et al. (2006) J Virol; 80 (3) : 1167-1180

• Other Product Names:

  PKB, PTK, CAKB, FAK2, PYK2, CADTK, FADK2, RAFTK, MAPTL, MTBT1, TAU, FADK 2, Focal adhesion kinase 2, PTK2B, Proline-rich tyrosine kinase 2 RAFTK

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  27886584

• Physical Properties:

  Liquid