PKCbeta (phospho Thr641) Antibody

• Background:

  Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-κ -B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-κ -B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR) -dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.

• NCBI Gene ID:

  5579

• Swiss Prot:

  P05771

• Accession Number:

  NP_002729.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  PKCbeta (Phospho-Thr641) antibody was raised against a peptide sequence around phosphorylation site of threonine 641 (E-L-T (p) -P-T) derived from Human PKCβ .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Apoptosis, Cancer, Neuroscience

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  82 kDa

• Specificity:

  This antibody detects endogenous levels of PKCβ only when phosphorylated at threonine 641.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  PRKCB

• NCBI Official Name:

  Protein kinase C, beta

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Zhang Y, et al. (2006) Mol Cell Biol ; 26: 6748-6761Castoria G, et al. (2004) Mol Cell Biol ; 24: 7643-7653Marcil J, et al. (1999) Biochem J ; 337:185-192Bornancin F, et al. (1996) Curr Biol ; 6:1114-1123.

• Other Product Names:

  PKCB, PRKCB1, PRKCB2, PKC-beta, IKBA, MAD3, NFKBI, PKCB

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  20127450

• Physical Properties:

  Liquid