P53 (phospho Ser15) Antibody

• Background:

  Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

• NCBI Gene ID:

  7157

• Swiss Prot:

  P04637

• Accession Number:

  NP_000537.3

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  P53 (Phospho-Ser15) antibody was raised against a peptide sequence around phosphorylation site of serine 15 (P-L-S (p) -Q-E) derived from Human p53.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Apoptosis, Cancer, Cell Cycle

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  53 kDa

• Specificity:

  This antibody detects endogenous level of p53 only whenphosphorylated at serine15.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  TP53

• NCBI Official Name:

  Tumor protein p53

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lin T, et al. (2005) Nat Cell Biol; 7 (2) : 165-71. Vega FM, et al. (2004) Mol Cell Biol; 24 (23) : 10366-80. Li J, et al. (2004) J Biol Chem; 279 (40) : 41275-9. Wang J, et al. (2004) J Biol Chem; 279 (38) : 39584-92.

• Other Product Names:

  P53, BCC7, LFS1, TRP53, MEK6, MKK6, PRKMK6, SKK3, Tumor suppressor p53, Phosphoprotein p53, Antigen NY-CO-13, TP53

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  120407068

• Physical Properties:

  Liquid