IkB a (phospho Ser32 / Ser36) Antibody

• Background:

  Inhibits the activity of dimeric NF-κ -B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.

• NCBI Gene ID:

  4792

• Swiss Prot:

  P25963

• Accession Number:

  NP_065390.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  IkB-a (Phospho-Ser32/Ser36) antibody was raised against a peptide sequence around phosphorylation site of serine 32/36 (H-D-S (p) -G-L-D-S (p) -M-K) derived from Human IkappaB-α .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Apoptosis, Cancer

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  39 kDa

• Specificity:

  This antibody detects endogenous level of IkappaB-α only when phosphorylated at serine 32/36.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  NFKBIA

• NCBI Official Name:

  Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Shieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823. Honda, R. et al. (1997) FEBS Lett. 420, 25-27. Hirao, A. et al. (2000) Science 287, 1824-1827. Tibbetts, R. S. et al. (1999) Genes Dev. 13, 152-157. Chehab, N. H. et al. (1999) Proc . Natl . Acad. Sci. USA 96, 13777-13782.

• Other Product Names:

  IKBA, MAD-3, NFKBI, ADDA, I-kappa-B-alpha, IKBA, NF-kappaB inhibitor alpha, NFKBIA

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  10092619

• Physical Properties:

  Liquid