HER2 (phospho Tyr877) Antibody

• Background:

  Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin.

• NCBI Gene ID:

  2064

• Swiss Prot:

  P04626

• Accession Number:

  NP_001005862.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  HER2 (Phospho-Tyr877) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 877 (T-E-Y (p) -H-A) derived from Human HER2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  185 kDa

• Specificity:

  This antibody detects endogenous level of HER2 only when phosphorylated at tyrosine 877.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  ERBB2

• NCBI Official Name:

  V-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Dittadi, R. et al. (2000) J. Natl. Cancer Inst. 92, 1443-1444. Muthuswamy, S. K. et al. (1999) Mol. Cell. Biol. 19, 6845-6857. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1500-1504.

• Other Product Names:

  NEU, NGL, HER2, TKR1, CD340, HER-2, MLN19, HER-2/neu, BHLHE39, C-erbB-2, ErbB2

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  54792098

• Physical Properties:

  Liquid