BAD (phospho Ser112) Antibody

• Background:

  The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

• NCBI Gene ID:

  572

• Swiss Prot:

  Q92934

• Accession Number:

  NP_004313.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  BAD (Phospho-Ser112) antibody was raised against a peptide sequence around phosphorylation site of serine 112 (H-S-S (p) -Y-P) derived from Human BAD.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Apoptosis, Cancer

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  23 kDa

• Specificity:

  This antibody detects endogenous level of BAD only when phosphorylated at serine 112.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

• Symbol:

  BAD

• NCBI Official Name:

  BCL2-associated agonist of cell death

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Zhang B, et al. (2004) . Mol Cell Biol. 24 (14) : 6205-6214. Rice PL, et al. (2003) . Cancer Res. 63 (3) : 616-620. Wang XQ, et al. (2001) . J Biol Chem. 276 (48) : 44504-44511.

• Other Product Names:

  BBC2, BCL2L8, ERBB, ERBB1, HER1, BBC6

• Tested Applications:

  WB, IHC

• Protein ID:

  10835069

• Physical Properties:

  Liquid