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GFP Antibody [4B10B2]

CAT: 0223-32-160Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0223-32-160Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
NCBI Gene ID
7011691
Swiss Prot
B6UPG7
Accession Number
YP_002302326.1
Host
Mouse
Reactivity
GFP
Immunogen
Ni-NTA purified full-length recombinant GFP-His expressed in E. Coli strain BL21 (DE3) .
Clonality
Monoclonal
Clone
4B10B2
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific
Concentration
Batch dependent
Buffer
Purified antibody in PBS containing 0.03% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
GFP monoclonal antibody can be stored at -20˚ C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG2a
Symbol
Gfp
NCBI Official Name
Green fluorescent protein
NCBI Organism
Neisseria gonorrhoeae
Background Reference 01
Dittman JS. Kaplan JM. Proc Natl Acad Sci U S A. 2006, Jul 25,103 (30) :11399-404. Epub 2006 Jul 14.
Background Reference 02
Cavalieri V. Bernardo MD. Spinelli G. Gene Expr Patterns. 2007, Jan,7 (1-2) :124-30. Epub 2006 Jun 14.
Background Reference 03
Arasada R. Son H. Ramalingam N. et al. Eur J Cell Biol. 2006, Sep,85 (9-10) :1059-68. Epub 2006 Jul 13.
Other Product Names
Green fluorescent protein
Tested Applications
ELISA, WB
Protein ID
211909965

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