RT25 Antibody

• Background :

  Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4.

• NCBI Gene ID :

  64432

• Swiss Prot :

  P82663

• Accession Number :

  P82663

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This RT25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-173 amino acids from the C-terminal region of human RT25.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Other

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  20 kDa

• Fragment :

  Rabbit Ig

• Symbol :

  MRPS25

• NCBI Official Name :

  28S ribosomal protein S25, mitochondrial

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :

• Background Reference 02 :

  Zhang, Z., et al. Genomics 81 (5) :468-480 (2003)

• Background Reference 03 :

  Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001) :

• Background Reference 04 :

  Cavdar Koc, E., et al. J. Biol. Chem. 276 (22) :19363-19374 (2001)

• Other Product Names :

  28S ribosomal protein S25, mitochondrial, MRP-S25, S25mt, MRPS25, RPMS25

• Tested Applications :

  WB, IHC-P, Flow

• Protein ID :

  13633894

• Physical Properties :

  Liquid