CTDSP1-V250 Antibody

• Background:

  CTDSP1 is a class 2C phosphatase with activity dependent on the conserved DxD motif. Expression of CTDSP1 inhibited activated transcription from several promoter-reporter gene constructs, but expression of a mutant lacking phosphatase activity enhanced transcription. Neuronal gene transcription is repressed in nonneuronal cells by the repressor element-1 (RE1) -silencing transcription factor/neuron-restrictive silencer factor (REST/NRSF; 600571) complex. REST/NRSF recruits SCPs to neuronal genes that contain RE1 elements, leading to neuronal gene silencing in nonneuronal cells. Phosphatase-inactive forms of SCP interfere with REST/NRSF function and promote neuronal differentiation of P19 stem cells. Likewise, small interfering RNA directed to the single Drosophila SCP unmasks neuronal gene expression in S2 cells. Thus, SCP activity is an evolutionarily conserved transcriptional regulator that acts globally to silence neuronal genes.

• NCBI Gene ID:

  58190

• Swiss Prot:

  Q9GZU7

• Accession Number:

  Q9GZU7

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This CTDSP1-V250 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-261 amino acids from the C-terminal region of human CTDSP1-V250.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  29 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  CTDSP1

• NCBI Official Name:

  Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Yeo, M., Lee, S.K., Lee, B., Ruiz, E.C., Pfaff, S.L. and Gill, G.N. Science 307 (5709) : 596-600 (2005) .

• Background Reference 02:

  Fernandes, A.O., Campagnoni, C.W., Kampf, K., Feng, J.M., Handley, V.W. J. Neurosci. Res. 75 (4) : 461-471 (2004) .

• Background Reference 03:

  Yeo, M., Lin, P.S., Dahmus, M.E. and Gill, G.N. J. Biol. Chem. 278 (28) : 26078-26085 (2003) .

• Other Product Names:

  Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1, Nuclear LIM interactor-interacting factor 3, NLI-IF, NLI-interacting factor 3, Small C-terminal domain phosphatase 1, SCP1, Small CTD phosphatase 1, CTDSP1, NIF3, NLIIF, SCP1

• Tested Applications:

  WB, IHC-P

• Protein ID:

  17865510

• Physical Properties:

  Liquid