PFKL Antibody

• Background:

  Phosphofructokinase (PFK), a major regulatory enzyme in all cells of the body, catalyzes the metabolism of sugar, and thereby is pivotal in the production of energy to maintain normal cell function. In human there are three structural loci controlling PFK: M (muscle), L (liver), and P (platelet) type subunits, which are variably expressed in different tissues; human diploid fibroblasts and leukocytes express all three genes. PFK, a tetramer formed by the random association of the products of two separate gene loci to form the five possible tetramers. PFKs of muscle and liver are homotetramers of the M and L subunits, respectively. Red cells have all five isozymes: M4, M3L, M2L2, ML3, and L4. PFK is an allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. PFK catalyzes the key controlling step of glycolytic pathway. PFK deficiency can present as mild to life-threatening episodic illness. A hallmark sign of this disease is intermittent dark urine, with the color of the urine ranging from orange to dark coffee-brown, which commonly develops following strenuous exercise. The mean red cell PFK is elevated in persons with Down syndrome.

• NCBI Gene ID:

  5211

• Swiss Prot:

  P17858

• Accession Number:

  P17858

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This PFKL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 669-699 amino acids from the C-terminal region of human PFKL.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  85 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  PFKL

• NCBI Official Name:

  ATP-dependent 6-phosphofructokinase, liver type {ECO:0000255|HAMAP-Rule:MF_03184}

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99 (26) :16899-16903 (2002) .

• Background Reference 02:

  Hattori, M., et al., Nature 405 (6784) :311-319 (2000) .

• Background Reference 03:

  Elson, A., et al., Genomics 7 (1) :47-56 (1990) .

• Background Reference 04:

  Levanon, D., et al., DNA 8 (10) :733-743 (1989) .

• Other Product Names:

  ATP-dependent 6-phosphofructokinase, liver type {ECO:0000255|HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184}, PFK-L, 27111 {ECO:0000255|HAMAP-Rule:MF_03184}, 6-phosphofructokinase type B, Phosphofructo-1-kinase isozyme B, PFK-B, Phosphohexokinase {ECO:0000255|HAMAP-Rule:MF_03184}, PFKL

• Tested Applications:

  WB, IHC-P

• Protein ID:

  134048493

• Physical Properties:

  Liquid