PLK3 Antibody

• Background :

  CNK, a member of the CDC5/Polo subfamily of Ser/Thr protein kinases, is involved in regulating M phase functions during the cell cycle. It may also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein. This membrane-associated protein binds to the calcium/integrin-binding protein (CIB) . This interaction probably occurs via the POLO-box domain. Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. This protein exhibits a short half-live. In cells of hematopoietic origin, CNK is strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor. Cytokine and cellular adhesion trigger CNK induction. CNK is thought to be phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis The protein contains 2 POLO box domains.

• NCBI Gene ID :

  1263

• Swiss Prot :

  Q9H4B4

• Accession Number :

  Q9H4B4

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  This PLK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 615-646 amino acids from the C-terminal region of human PLK3.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Signal Transduction

• Purification :

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration :

  Batch dependent

• Homology :

  Predicted species reactivity based on immunogen sequence: Rat

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  72 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  PLK3

• NCBI Official Name :

  Serine/threonine-protein kinase PLK3

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Holtrich, U., et al., Oncogene 19 (42) :4832-4839 (2000) .

• Background Reference 02 :

  Ouyang, B., et al., Oncogene 18 (44) :6029-6036 (1999) .

• Background Reference 03 :

  Ouyang, B., et al., J. Biol. Chem. 272 (45) :28646-28651 (1997) .

• Background Reference 04 :

  Li, B., et al., J. Biol. Chem. 271 (32) :19402-19408 (1996) .

• Other Product Names :

  Serine/threonine-protein kinase PLK3, Cytokine-inducible serine/threonine-protein kinase, FGF-inducible kinase, Polo-like kinase 3, PLK-3, Proliferation-related kinase, PLK3, CNK, FNK, PRK

• Tested Applications :

  WB

• Protein ID :

  51338822

• Physical Properties :

  Liquid