TRIB1 Antibody

• Background:

  TRIB1 antibody, also called gprotein coupled receptor induced, skip1, gig2, c8fwATP, functions in binding protein binding, protein kinase inhibitor activity, and protein serine/threonine kinase activities. Overexpression of TRIB1 in HeLa cells repressed the basal activity of the IL8 promoter by inhibiting AP1 activity. Overexpression of TRIB1 inhibited oncogenic Ras -driven AP1 activation and MEKK1-mediated AP1 activation. ERK activation was enhanced by TRIB1. Coimmunoprecipitation and yeast 2-hybrid assays showed that MEK1 interacted with both TRIB1 and TRIB3, and MKK4 interacted specifically with TRIB1. Cotransfection of MKK4 enhanced the level of TRIB1, indicating that the TRIB-MAPKK interaction stabilized TRIB1.

• NCBI Gene ID:

  10221

• Swiss Prot:

  Q96RU8

• Accession Number:

  Q96RU8

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This TRIB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the C-terminal region of human TRIB1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  41 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:2000

• Symbol:

  TRIB1

• NCBI Official Name:

  Tribbles homolog 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kiss-Toth, E., et al., J. Biol. Chem. 279 (41) :42703-42708 (2004) .

• Background Reference 02:

  Wu, M., et al., J. Biol. Chem. 278 (29) :27072-27079 (2003) .

• Background Reference 03:

  Wilkin, F., et al., Eur. J. Biochem. 248 (3) :660-668 (1997) .

• Other Product Names:

  HGNC:16891

• Tested Applications:

  WB

• Protein ID:

  83305929

• Physical Properties:

  Liquid