ABL1 Antibody

• Background:

  The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t (9;22) translocation results in the head-to-tail fusion of the BCR and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.

• NCBI Gene ID:

  25

• Swiss Prot:

  P00519

• Accession Number:

  P00519

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This ABL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-260 amino acids from the N-terminal region of human ABL1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  123 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  ABL1

• NCBI Official Name:

  Tyrosine-protein kinase ABL1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Donaldson, L.W., et al., Proc. Natl. Acad. Sci. U.S.A. 99 (22) :14053-14058 (2002) .

• Background Reference 02:

  Pisabarro, M.T., et al., J. Mol. Biol. 281 (3) :513-521 (1998) .

• Background Reference 03:

  Nam, H.J., et al., Structure 4 (9) :1105-1114 (1996) .

• Background Reference 04:

  Chissoe, S.L., et al., Genomics 27 (1) :67-82 (1995) .

• Other Product Names:

  Tyrosine-protein kinase ABL1, Abelson murine leukemia viral oncogene homolog 1, Abelson tyrosine-protein kinase 1, Proto-oncogene c-Abl, p150, ABL1, ABL, JTK7

• Tested Applications:

  WB

• Protein ID:

  85681908

• Physical Properties:

  Liquid