AlaRS Antibody

• Background:

  The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes.Class II tRNA synthases evolved early in evolution and are highly conserved.This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein.tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons.They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.

• NCBI Gene ID:

  16

• Swiss Prot:

  P49588

• Accession Number:

  P49588

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This AlaRS (AARS) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-91 amino acids from the N-terminal region of human AlaRS (AARS) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: C. Elegans, Drosophila, Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  107 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  AARS

• NCBI Official Name:

  Alanine--tRNA ligase, cytoplasmic {ECO:0000255|HAMAP-Rule:MF_03133}

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Shiba, K., Biochemistry 34 (33), 10340-10349 (1995)

• Background Reference 02:

  Ripmaster, T.L., Proc. Natl. Acad. Sci. U.S.A. 92 (11), 4932-4936 (1995)

• Background Reference 03:

  Sang Lee, J., Cell 127 (3), 635-648 (2006)

• Other Product Names:

  Alanine--tRNA ligase, cytoplasmic {ECO:0000255|HAMAP-Rule:MF_03133}, 6117 {ECO:0000255|HAMAP-Rule:MF_03133}, Alanyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_03133}, AlaRS {ECO:0000255|HAMAP-Rule:MF_03133}, Renal carcinoma antigen NY-REN-42, AARS {ECO:0000255|HAMAP-Rule:MF_03133}

• Tested Applications:

  WB, IHC-P

• Protein ID:

  115502460

• Physical Properties:

  Liquid