PICK1 Antibody

CAT: 0223-62-642Size: 400 µLDry Ice: NoHazardous: No
CAT#:0223-62-642Size:400 µL
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Background
PDZ domain, but not the AH domain, of PICK1 interacts with the C termini of the GTP-bound forms of ADP-ribosylation factor-1 (ARF1) and ARF3. The interactions with ARF5 and ARF6 are weak, suggesting that the PICK1 interaction is specific for class I ARFs and that it may regulate Golgi-to-endoplasmic reticulum vesicle transport. The PDZ domain of rat Pick1 interacts with the last 10 amino acids of the short C-terminal alternative splice variants of AMPA receptor subunits. It has thus been proposed that E-S-V/I-K-I, a sequence found in these 10 amino acids, is a novel PDZ-binding motif. PRKCA phosphorylates Pick1 efficiently but binds Pick1 in both the phosphorylated and unphosphorylated states. Consistent with a neuronal role for PICK1, the mouse homolog interacts with mouse AMPA glutamate receptors and colocalizes at excitatory synapses in the brain. Metabotropic glutamate receptor-7 (mGluR7) localizes specifically to presynaptic active zones. The extreme C-terminal 3 amino acids of mGluR7 have been shown to interact with the PDZ domain of PICK1. Immunofluorescence microscopy demonstrated that both proteins are localized at excitatory synapses in hippocampal neurons, with clustering of mGluR7 at synapses requires PICK1 C-terminal PDZ-binding residues. Mutant mGluR7 lacking the PDZ-binding residues localized diffusely along axons rather than at the synapse, suggesting a role for Pick1 as a scaffolding molecule at presynaptic sites.
NCBI Gene ID
9463
Swiss Prot
Q9NRD5
Accession Number
Q9NRD5
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
This PICK1 (PRKCABP) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-316 amino acids from the C-terminal region of human PICK1 (PRKCABP) .
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Neuroscience, Signal Transduction
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Rat
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
47 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
PICK1
NCBI Official Name
PRKCA-binding protein
NCBI Organism
Homo sapiens
Other Product Names
PRKCA-binding protein, Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein, PICK1, PRKCABP
Tested Applications
WB
Protein ID
22095990
Physical Properties
Liquid

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