AMPK gamma Antibody

• Background:

  PRKAG1 is a regulatory subunit of the AMP-activated protein kinase (AMPK) . AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK.

• NCBI Gene ID:

  5571

• Swiss Prot:

  P54619

• Accession Number:

  P54619

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This AMPK gamma (PRKAG1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-295 amino acids from the Central region of human AMPK gamma (PRKAG1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Pig

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  38 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  PRKAG1

• NCBI Official Name:

  5'-AMP-activated protein kinase subunit gamma-1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Minokoshi, Y., et al., Nature 428 (6982) :569-574 (2004) .

• Background Reference 02:

  Hamilton, S.R., et al., FEBS Lett. 500 (3) :163-168 (2001) .

• Background Reference 03:

  Zidovetzki, R., et al., AIDS Res. Hum. Retroviruses 14 (10) :825-833 (1998) .

• Background Reference 04:

  Reinton, N., et al., Genomics 49 (2) :290-297 (1998) .

• Other Product Names:

  5'-AMP-activated protein kinase subunit gamma-1, AMPK gamma1, AMPK subunit gamma-1, AMPKg, PRKAG1

• Tested Applications:

  WB, IHC-P

• Protein ID:

  1703037

• Physical Properties:

  Liquid