IKK gamma Antibody

• Background:

  IKK gamma Antibody: Nuclear factor kappa B (NF-κ B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κ B is associated with Iκ B proteins in the cell cytoplasm, which inhibit NF-κ B activity. The Iκ B kinase (IKKα and IKKβ ) phosphorylates IkB and mediates NF-κ B activation. A novel molecule in the IKK complex was recently identified and termed IKKγ /NEMO/FIP3/IKKAP1. IKKγ interacts with IKKβ and is required for the activation of IKK complex and NF-κ B by LPS, PMA, TNF, and IL-1 stimulation. FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-κ B activation.

• NCBI Gene ID:

  8517

• Swiss Prot:

  Q9Y6K9

• Accession Number:

  AF074382

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  IKK gamma Antibody is DEAE purified.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  1 mg/ml

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (94%)

• Buffer:

  IKK gamma Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  IKK gamma antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  52 kDa

• Fragment:

  IgG

• Specificity:

  IKK gamma has no cross response to IKK alpha or IKK beta.

• Symbol:

  IKBKG

• Positive Control 2:

  Cat. No. 17-001 - HeLa Cell Slide

• NCBI Official Name:

  Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-γ is an essential regulatory subunit of the Iκ B kinase complex. Nature 1998;395 (6699) :297-300

• Background Reference 02:

  Yamaoka S, Courtois G, Bessia C, Whiteside ST, Weil R, Agou F, Kirk HE, Kay RJ, Israel A. Complementation cloning of NEMO, a component of the Iκ B kinase complex essential for NF-κ B activation. Cell. 1998;93 (7) :1231-40.

• Background Reference 03:

  Li Y, Kang J, Friedman J, Tarassishin L, Ye J, Kovalenko A, Wallach D, Horwitz MS. Identification of a cell protein (FIP-3) as a modulator of NF-κ B activity and as a target of an adenovirus inhibitor of tumor necrosis factor α -induced apoptosis. Proc Natl Acad Sci U S A 1999;96 (3) :1042-7

• Background Reference 04:

  Mercurio F, Murray BW, Shevchenko A, Bennett BL, Young DB, Li JW, Pascual G, Motiwala A, Zhu H, Mann M, Manning AM. Iκ B kinase (IKK) -associated protein 1, a common component of the heterogeneous IKK complex. Mol Cell Biol. 1999;19 (2) :1526-38.

• Other Product Names:

  IKK gamma Antibody: IP, IP1, IP2, FIP3, IPD2, NEMO, FIP-3, Fip3p, AMCBX1, ZC2HC9, IKK-gamma, NF-kappa-B essential modulator

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  3641279

• Physical Properties:

  Liquid