SETD8 Antibody

• Background:

  SETD8 is a histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20' monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. The protein mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It is required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. It is also involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones.

• NCBI Gene ID:

  387893

• Swiss Prot:

  Q9NQR1

• Accession Number:

  Q9NQR1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This SETD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from the Central region of human SETD8.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  43 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  SETD8

• NCBI Official Name:

  N-lysine methyltransferase SETD8

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kalakonda, N., Oncogene 27 (31), 4293-4304 (2008)

• Background Reference 02:

  Houston, S.I., J. Biol. Chem. 283 (28), 19478-19488 (2008)

• Other Product Names:

  N-lysine methyltransferase SETD8, 211-, H4-K20-HMTase SETD8, Histone-lysine N-methyltransferase SETD8, Lysine N-methyltransferase 5A, PR/SET domain-containing protein 07, PR-Set7, PR/SET07, SET domain-containing protein 8, SETD8, KMT5A, PRSET7, SET07, SET8

• Tested Applications:

  WB, IHC-P, Flow

• Protein ID:

  25091219

• Physical Properties:

  Liquid