ATP6V0A4 Antibody

• Background:

  This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing.

• NCBI Gene ID:

  50617

• Swiss Prot:

  Q9HBG4

• Accession Number:

  Q9HBG4

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This ATP6V0A4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-261 amino acids from the Central region of human ATP6V0A4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  96 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  ATP6V0A4

• NCBI Official Name:

  V-type proton ATPase 116 kDa subunit a isoform 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Andreucci, E., et al. Pediatr. Nephrol. 24 (11) :2147-2153 (2009)

• Background Reference 02:

  Hinton, A., et al. J. Biol. Chem. 284 (24) :16400-16408 (2009)

• Background Reference 03:

  Su, Y., et al. Am. J. Physiol. Renal Physiol. 295 (4), F950-F958 (2008) :

• Background Reference 04:

  Norgett, E.E., et al. J. Biol. Chem. 282 (19) :14421-14427 (2007)

• Other Product Names:

  V-type proton ATPase 116 kDa subunit a isoform 4, V-ATPase 116 kDa isoform a4, Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4, Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform, ATP6V0A4, ATP6N1B, ATP6N2

• Tested Applications:

  WB, Flow

• Protein ID:

  308153516

• Physical Properties:

  Liquid