DLAT Antibody

• Background:

  DLAT encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC) . PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC) . In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure.

• NCBI Gene ID:

  1737

• Swiss Prot:

  P10515

• Accession Number:

  P10515

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This DLAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 579-607 amino acids from the C-terminal region of human DLAT.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  69 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  DLAT

• NCBI Official Name:

  Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Trynka, G., et al. Gut 58 (8) :1078-1083 (2009)

• Background Reference 02:

  Lleo, A., et al. Hepatology 49 (3) :871-879 (2009)

• Background Reference 03:

  Korotchkina, L.G., et al. FEBS Lett. 582 (3) :468-472 (2008)

• Other Product Names:

  Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, PBC, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, M2 antigen complex 70 kDa subunit, Pyruvate dehydrogenase complex component E2, PDC-E2, PDCE2, DLAT, DLTA

• Tested Applications:

  WB, IHC-P, Flow

• Protein ID:

  215274207

• Physical Properties:

  Liquid