HSPA5 Antibody

• Background:

  When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER) . Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.

• NCBI Gene ID:

  3309

• Swiss Prot:

  P11021

• Accession Number:

  P11021

• Host:

  Rabbit

• Reactivity:

  Human, Zebrafish

• Immunogen:

  This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-289 amino acids from the Central region of human HSPA5.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  72 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  HSPA5

• NCBI Official Name:

  78 kDa glucose-regulated protein

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Zhao, C., et al. J. Med. Virol. 82 (1) :14-22 (2010) Zhuang, L., et al. Mod. Pathol. 23 (1) :45-53 (2010) Arnaudeau, S., et al. Proteomics 9 (23) :5316-5327 (2009)

• Other Product Names:

  78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca (2+) -binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP, HSPA5, GRP78

• Tested Applications:

  WB, IHC-P, IF

• Protein ID:

  14916999

• Physical Properties:

  Liquid