HSPA5 Antibody

• Background :

  When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER) . Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.

• NCBI Gene ID :

  3309

• Swiss Prot :

  P11021

• Accession Number :

  P11021

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541-568 amino acids from the C-terminal region of human HSPA5.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Other

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Homology :

  Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat, Xenopus

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  72 kDa

• Fragment :

  Rabbit Ig

• Symbol :

  HSPA5

• NCBI Official Name :

  78 kDa glucose-regulated protein

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Zhao, C., et al. J. Med. Virol. 82 (1) :14-22 (2010)

• Background Reference 02 :

  Zhuang, L., et al. Mod. Pathol. 23 (1) :45-53 (2010)

• Background Reference 03 :

  Arnaudeau, S., et al. Proteomics 9 (23) :5316-5327 (2009)

• Other Product Names :

  78 kDa glucose-regulated protein, GRP-78, Endoplasmic reticulum lumenal Ca (2+) -binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP, HSPA5, GRP78

• Tested Applications :

  WB, IHC-P, Flow

• Protein ID :

  14916999

• Physical Properties :

  Liquid