PCNA Antibody

• Background:

  PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.

• NCBI Gene ID:

  5111

• Swiss Prot:

  P12004

• Accession Number:

  P12004

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-261 amino acids from the C-terminal region of human PCNA.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Hamster, Monkey, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  29 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  PCNA

• NCBI Official Name:

  Proliferating cell nuclear antigen

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Wang, Y., J. Cell. Biochem. 106 (3), 409-413 (2009) Maga, G., Proc. Natl. Acad. Sci. U.S.A. 105 (52), 20689-20694 (2008) Acharya, N., Proc. Natl. Acad. Sci. U.S.A. 105 (46), 17724-17729 (2008)

• Other Product Names:

  Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA

• Tested Applications:

  WB, IHC-P, IF

• Protein ID:

  129694

• Physical Properties:

  Liquid