MARCKS Antibody

• Background:

  MARCKS is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.

• NCBI Gene ID:

  4082

• Swiss Prot:

  P29966

• Accession Number:

  P29966

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This MARCKS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-271 amino acids from the C-terminal region of human MARCKS.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  32 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  MARCKS

• NCBI Official Name:

  Myristoylated alanine-rich C-kinase substrate

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Rauch, M.E., et al., J. Biol. Chem. 277 (16) :14068-14076 (2002) .

• Background Reference 02:

  Aderem, A., Biochem. Soc. Trans. 23 (3) :587-591 (1995) .

• Background Reference 03:

  Rao, P.H., et al., Cytogenet. Cell Genet. 66 (4) :272-273 (1994) .

• Background Reference 04:

  Taniguchi, H., et al., J. Biol. Chem. 268 (14) :9960-9963 (1993) .

• Other Product Names:

  Myristoylated alanine-rich C-kinase substrate, MARCKS, Protein kinase C substrate, 80 kDa protein, light chain, 80K-L protein, PKCSL, MARCKS, MACS, PRKCSL

• Tested Applications:

  WB

• Protein ID:

  76803798

• Physical Properties:

  Liquid