Ribophorin Antibody

• Background:

  Ribophorins 1 and 2 are abundant and highly conserved glycoproteins residing in the endoplasic reticulum, that participate in ribosome binding. Mammalian oligosaccharyltransferase activity is associated with a protein complex composed of RPN1, RPN2, and an oligosaccharyltransferase protein. RPN1 is a component of the proteasome base. The ubiquitin-like (UBL) domain of recombinant Rad23 interacts with proteasomes through the leucine-rich repeat domain of RPN1. The RPN1 gene maps to chromosome 3 in somatic cell hybrids, and the RPN2 gene maps to chromosome 20 by in situ hybridization.

• NCBI Gene ID:

  6184

• Swiss Prot:

  P04843

• Accession Number:

  P04843

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This Ribophorin (RPN1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-561 amino acids from the C-terminal region of human Ribophorin (RPN1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Monkey, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  69 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  RPN1

• NCBI Official Name:

  Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Fu, J., et al., J. Biol. Chem. 275 (6) :3984-3990 (2000) . Pekarsky, Y., et al., Cancer Res. 57 (18) :3914-3919 (1997) . Crimaudo, C., et al., EMBO J. 6 (1) :75-82 (1987) .

• Other Product Names:

  Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, Ribophorin I, RPN-I, Ribophorin-1, RPN1

• Tested Applications:

  WB

• Protein ID:

  132559

• Physical Properties:

  Liquid