NDC80 Antibody

• Background:

  Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

• NCBI Gene ID:

  10403

• Swiss Prot:

  O14777

• Accession Number:

  O14777

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This NDC80 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 508-539 amino acids from human NDC80.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  74 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:2000

• Symbol:

  NDC80

• NCBI Official Name:

  Kinetochore protein NDC80 homolog

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Chen Y., et al.Mol. Cell. Biol. 17:6049-6056 (1997) .

• Background Reference 02:

  Chen Y., et al.J. Biol. Chem. 272:24081-24087 (1997) .

• Background Reference 03:

  Zheng L., et al.Mol. Cell. Biol. 19:5417-5428 (1999) .

• Background Reference 04:

  Zheng L., et al.Mol. Cell. Biol. 20:3529-3537 (2000) .

• Other Product Names:

  Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HEC, NDC80, HEC, HEC1, KNTC2

• Tested Applications:

  WB

• Physical Properties:

  Liquid