BAAT Antibody

• Background:

  Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids) . May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids) . May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs.

• NCBI Gene ID:

  570

• Swiss Prot:

  Q14032

• Accession Number:

  Q14032

• Host:

  Rabbit

• Reactivity:

  Rat

• Immunogen:

  This BAAT antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 63-95 amino acids from human BAAT.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  46 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:2000

• Symbol:

  BAAT

• NCBI Official Name:

  Bile acid-CoA:amino acid N-acyltransferase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Falany C.N., et al.J. Biol. Chem. 269:19375-19379 (1994) .

• Background Reference 02:

  Ebert L., et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

• Background Reference 03:

  Humphray S.J., et al.Nature 429:369-374 (2004) .

• Background Reference 04:

  Mural R.J., et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

• Other Product Names:

  Bile acid-CoA:amino acid N-acyltransferase, BACAT, BAT, 2.3.1.65, Glycine N-choloyltransferase, Long-chain fatty-acyl-CoA hydrolase, 3.1.2.2, BAAT

• Tested Applications:

  WB

• Protein ID:

  74739811

• Physical Properties:

  Liquid