BAAT Antibody

Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids) . May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids) . May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs.

570

Q14032

Q14032

Rat

This BAAT antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 63-95 amino acids from human BAAT.

Unconjugated

Primary Antibodies

Cancer, Obesity, Signal Transduction

This antibody is purified through a protein A column, followed by peptide affinity purification.

Batch dependent

Supplied in PBS with 0.09% (W/V) sodium azide.

None

Blue Ice

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

46 kDa

Rabbit Ig

For WB starting dilution is: 1:2000

BAAT

Bile acid-CoA:amino acid N-acyltransferase

Homo sapiens

Falany C.N., et al.J. Biol. Chem. 269:19375-19379 (1994) .

Ebert L., et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

Humphray S.J., et al.Nature 429:369-374 (2004) .

Mural R.J., et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

Bile acid-CoA:amino acid N-acyltransferase, BACAT, BAT, 2.3.1.65, Glycine N-choloyltransferase, Long-chain fatty-acyl-CoA hydrolase, 3.1.2.2, BAAT

WB

74739811