E1 Ubiquitin Antibody

• Background:

  UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR.

• NCBI Gene ID:

  7317

• Swiss Prot:

  P22314

• Accession Number:

  P22314

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This E1 Ubiquitin (UBE1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1026-1058 amino acids from the C-terminal region of human E1 Ubiquitin (UBE1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Rabbit, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  118 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  UBA1

• NCBI Official Name:

  Ubiquitin-like modifier-activating enzyme 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ayusawa, D., et al., Cell Struct. Funct. 17 (2) :113-122 (1992) . Handley, P.M., et al., Proc. Natl. Acad. Sci. U.S.A. 88 (1) :258-262 (1991) . Kudo, M., et al., Exp. Cell Res. 192 (1) :110-117 (1991) . Zacksenhaus, E., et al., Cytogenet. Cell Genet. 53 (1) :20-22 (1990) . Zacksenhaus, E., et al., EMBO J. 9 (9) :2923-2929 (1990) .

• Other Product Names:

  Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1, UBA1, A1S9T, UBE1

• Tested Applications:

  WB, IHC-P

• Protein ID:

  24418865

• Physical Properties:

  Liquid