Ubiquitin Antibody

• Background:

  This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

• NCBI Gene ID:

  7316

• Swiss Prot:

  P0CG48

• Accession Number:

  P0CG48

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This Ubiquitin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-66 amino acids of human Ubiquitin.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Neuroscience

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Chicken, Yeast, Xenopus, Bovine, C. Elegans, Drosophila

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  77 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  UBC

• NCBI Official Name:

  Polyubiquitin-C

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Chan, Y.L., et al., Biochem. Biophys. Res. Commun. 215 (2) :682-690 (1995) .

• Background Reference 02:

  Cook, W.J., et al., J. Mol. Biol. 236 (2) :601-609 (1994) .

• Background Reference 03:

  Hubbard, M.J., et al., Biochim. Biophys. Acta 1200 (2) :191-196 (1994) .

• Background Reference 04:

  Wajih, N., et al., Protein Seq. Data Anal. 5 (1) :31-32 (1992) .

• Other Product Names:

  Polyubiquitin-C, Ubiquitin, UBC

• Tested Applications:

  WB, IHC-P, IF

• Protein ID:

  391358178

• Physical Properties:

  Liquid