BBS5 Antibody

• Background:

  The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8 (GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for BBSome complex ciliary localization but not for the proper complex assembly.

• NCBI Gene ID:

  129880

• Swiss Prot:

  Q8N3I7

• Accession Number:

  Q8N3I7

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This BBS5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 108-141 amino acids from the Central region of human BBS5.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Monkey, Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  39 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  BBS5

• NCBI Official Name:

  Bardet-Biedl syndrome 5 protein

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Li J.B., et al.Cell 117:541-552 (2004) .

• Background Reference 02:

  Bechtel S., et al.BMC Genomics 8:399-399 (2007) .

• Background Reference 03:

  Hillier L.W., et al.Nature 434:724-731 (2005) .

• Background Reference 04:

  Mural R.J., et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

• Other Product Names:

  Bardet-Biedl syndrome 5 protein, BBS5

• Tested Applications:

  WB

• Protein ID:

  74750959

• Physical Properties:

  Liquid