P100 Antibody

• Background:

  The nuclear protein p100 is a proliferation-associated protein whose expression is restricted to cell cycle phases S, G2, and M. Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. The full-length cDNA encodes a 747-amino acid protein with a putative ATP/GTP-binding site motif. RT-PCR analysis demonstrated strong expression of in lung carcinoma cell lines but not in normal lung tissues. Expression was also found in adult placenta, skeletal muscle, thymus, testis, and small intestine and in fetal brain, liver, and kidney. P100 is also correlated to cancer prognosis.

• NCBI Gene ID:

  22974

• Swiss Prot:

  Q9ULW0

• Accession Number:

  Q9ULW0

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This P100 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-382 amino acids from human P100.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  86 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  TPX2

• NCBI Official Name:

  Targeting protein for Xklp2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ota, T., et al., Nat. Genet. 36 (1) :40-45 (2004) .

• Background Reference 02:

  Heidebrecht, H.J., et al., Mol. Cancer Res. 1 (4) :271-279 (2003) .

• Background Reference 03:

  Garrett, S., et al., Curr. Biol. 12 (23) :2055-2059 (2002) .

• Background Reference 04:

  Gruss, O.J., et al., Nat. Cell Biol. 4 (11) :871-879 (2002) .

• Other Product Names:

  Targeting protein for Xklp2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL-2, Hepatocellular carcinoma-associated antigen 519, Hepatocellular carcinoma-associated antigen 90, Protein fls353, Restricted expression proliferation-associated protein 100, p100, TPX2, C20orf1, C20orf2, DIL2, HCA519

• Tested Applications:

  WB, IHC-P

• Protein ID:

  13124096

• Physical Properties:

  Liquid