GTF2F2 Antibody

• Background:

  TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.

• NCBI Gene ID:

  2963

• Swiss Prot:

  P13984

• Accession Number:

  P13984

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This GTF2F2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-172 amino acids from the Central region of human GTF2F2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  28 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  GTF2F2

• NCBI Official Name:

  General transcription factor IIF subunit 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Yang, A., et al. Biochemistry 48 (9) :1964-1974 (2009)

• Background Reference 02:

  Matsuoka, S., et al. Science 316 (5828) :1160-1166 (2007)

• Background Reference 03:

  Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345 (3) :1022-1032 (2006)

• Background Reference 04:

  Le, T.T., et al. J. Biochem. 138 (3) :215-224 (2005)

• Other Product Names:

  General transcription factor IIF subunit 2, ATP-dependent helicase GTF2F2, General transcription factor IIF 30 kDa subunit, Transcription initiation factor IIF subunit beta, TFIIF-beta, Transcription initiation factor RAP30, GTF2F2, RAP30

• Tested Applications:

  WB

• Protein ID:

  464519

• Physical Properties:

  Liquid