GTF2E2 Antibody

• Background:

  GTF2E2 belongs to the TFIIE beta subunit family.It recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

• NCBI Gene ID:

  2961

• Swiss Prot:

  P29084

• Accession Number:

  NP_002086

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2E2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  293T Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store GTF2E2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  33 kDa

• Applications Notes:

  GTF2E2 antibody can be used for detection of GTF2E2 by ELISA at 1:312500. GTF2E2 antibody can be used for detection of GTF2E2 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  GTF2E2

• NCBI Official Name:

  General transcription factor IIE, polypeptide 2, beta 34kDa

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Watanabe, T., (2003) Mol. Cell. Biol. 23 (8), 2914-2926.

• Other Product Names:

  GTF2E2, FE, TF2E2, TFIIE-B

• Tested Applications:

  ELISA, WB

• Protein ID:

  4504195

• Physical Properties:

  Liquid