KIRREL Antibody

• Background:

  NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762) . The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]) .

• NCBI Gene ID:

  55243

• Swiss Prot:

  Q96J84

• Accession Number:

  Q96J84

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This KIRREL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 505-534 amino acids from the C-terminal region of human KIRREL.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  84 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  KIRREL

• NCBI Official Name:

  Kin of IRRE-like protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Machuca, E., et al. J. Am. Soc. Nephrol. 21 (7) :1209-1217 (2010)

• Background Reference 02:

  Wagner, M.C., et al. J. Biol. Chem. 283 (51) :35579-35589 (2008)

• Background Reference 03:

  Hartleben, B., et al. J. Biol. Chem. 283 (34) :23033-23038 (2008)

• Background Reference 04:

  Harita, Y., et al. J. Biol. Chem. 283 (14) :9177-9186 (2008)

• Other Product Names:

  Kin of IRRE-like protein 1, Kin of irregular chiasm-like protein 1, Nephrin-like protein 1, KIRREL, KIRREL1, NEPH1

• Tested Applications:

  WB

• Protein ID:

  54036152

• Physical Properties:

  Liquid