RPL22 Antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2.

6146

P35268

P35268

Human, Mouse

This RPL22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the C-terminal region of human RPL22.

Unconjugated

Primary Antibodies

Other

This antibody is purified through a protein A column, followed by peptide affinity purification.

Batch dependent

Predicted species reactivity based on immunogen sequence: Chicken, Monkey, Pig, Rat, Xenopus

Supplied in PBS with 0.09% (W/V) sodium azide.

None

Blue Ice

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

15 kDa

Rabbit Ig

For WB starting dilution is: 1:2000

RPL22

60S ribosomal protein L22

Homo sapiens

Houmani, J.L., et al. J. Virol. 83 (19) :9844-9853 (2009)

Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28 (23) :7050-7065 (2008)

Fok, V., et al. RNA 12 (5) :872-882 (2006)

Nakao, K., et al. Otolaryngol Head Neck Surg 134 (4) :639-645 (2006)

60S ribosomal protein L22, EBER-associated protein, EAP, Epstein-Barr virus small RNA-associated protein, Heparin-binding protein HBp15, RPL22

WB