Mouse Il27 Antibody

CAT: 0223-60-460Size: 400 µLDry Ice: NoHazardous: No
CAT#:0223-60-460Size:400 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Cytokine with pro-and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17) . It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21-and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL-27 subunit p28 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9.
NCBI Gene ID
246779
Swiss Prot
Q8K3I6
Accession Number
Q8K3I6
Host
Rabbit
Reactivity
Mouse
Immunogen
This Mouse Il27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-150 amino acids from the Central region of mouse Il27.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Immunology
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
27 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
Il27
NCBI Official Name
Interleukin-27 subunit alpha
NCBI Organism
Mus musculus
Background Reference 01
Li, J.J., et al. J. Immunol. 185 (7) :4401-4409 (2010)
Background Reference 02
Zhang, J., et al. J. Biol. Chem. 285 (28) :21269-21281 (2010)
Background Reference 03
Baker, B.J., et al. Glia 58 (9) :1082-1093 (2010)
Background Reference 04
Murugaiyan, G., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (25) :11495-11500 (2010)
Other Product Names
Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A, IL27-A, p28, Il27, Il27a
Tested Applications
WB
Physical Properties
Liquid

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