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ATP6V1F Antibody

CAT: 0223-60-349Size: 400 µLDry Ice: NoHazardous: No
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CAT#:0223-60-349Size:400 µL
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Background
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein.
NCBI Gene ID
9296
Swiss Prot
Q16864
Accession Number
Q16864
Host
Rabbit
Reactivity
Human
Immunogen
This ATP6V1F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-111 amino acids from the C-terminal region of human ATP6V1F.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Obesity, Signal Transduction
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Bovine
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
13 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
ATP6V1F
NCBI Official Name
V-type proton ATPase subunit F
NCBI Organism
Homo sapiens
Background Reference 01
Supino, R., et al. Ann. N. Y. Acad. Sci. 1171, 606-616 (2009) :
Background Reference 02
Smith, A.N., et al. J. Bioenerg. Biomembr. 40 (4) :371-380 (2008)
Background Reference 03
Morel, N. Biol. Cell 95 (7) :453-457 (2003)
Background Reference 04
Smith, A.N., et al. Mol. Cell 12 (4) :801-803 (2003)
Other Product Names
V-type proton ATPase subunit F, V-ATPase subunit F, V-ATPase 14 kDa subunit, Vacuolar proton pump subunit F, ATP6V1F, ATP6S14, VATF
Tested Applications
WB
Protein ID
126302612
Physical Properties
Liquid

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