ATP6V0C Antibody

• Background:

  ATP6V0C is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. ATP6V0C encodes the V0 subunit c.

• NCBI Gene ID:

  527

• Swiss Prot:

  P27449

• Accession Number:

  P27449

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This ATP6V0C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-126 amino acids from the C-terminal region of human ATP6V0C.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: C. Elegans, Bovine, Drosophila, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  16 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  ATP6V0C

• NCBI Official Name:

  V-type proton ATPase 16 kDa proteolipid subunit

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  O'Callaghan, K.M., et al. J. Biol. Chem. 285 (1) :381-391 (2010)

• Background Reference 02:

  You, H., et al. Cancer Lett. 280 (1) :110-119 (2009)

• Background Reference 03:

  Lee, I., et al. J. Biol. Chem. 279 (51) :53007-53014 (2004)

• Background Reference 04:

  Morel, N. Biol. Cell 95 (7) :453-457 (2003)

• Other Product Names:

  V-type proton ATPase 16 kDa proteolipid subunit, V-ATPase 16 kDa proteolipid subunit, Vacuolar proton pump 16 kDa proteolipid subunit, ATP6V0C, ATP6C, ATP6L, ATPL

• Tested Applications:

  WB

• Protein ID:

  137479

• Physical Properties:

  Liquid