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ATP6V0C Antibody

CAT: 0223-55-198Size: 400 µLDry Ice: NoHazardous: No
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CAT#:0223-55-198Size:400 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
ATP6V0C is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. ATP6V0C encodes the V0 subunit c.
NCBI Gene ID
527
Swiss Prot
P27449
Accession Number
P27449
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
This ATP6V0C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-126 amino acids from the C-terminal region of human ATP6V0C.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Signal Transduction
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: C. Elegans, Bovine, Drosophila, Rat
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
16 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
ATP6V0C
NCBI Official Name
V-type proton ATPase 16 kDa proteolipid subunit
NCBI Organism
Homo sapiens
Background Reference 01
O'Callaghan, K.M., et al. J. Biol. Chem. 285 (1) :381-391 (2010)
Background Reference 02
You, H., et al. Cancer Lett. 280 (1) :110-119 (2009)
Background Reference 03
Lee, I., et al. J. Biol. Chem. 279 (51) :53007-53014 (2004)
Background Reference 04
Morel, N. Biol. Cell 95 (7) :453-457 (2003)
Other Product Names
V-type proton ATPase 16 kDa proteolipid subunit, V-ATPase 16 kDa proteolipid subunit, Vacuolar proton pump 16 kDa proteolipid subunit, ATP6V0C, ATP6C, ATP6L, ATPL
Tested Applications
WB
Protein ID
137479
Physical Properties
Liquid